0200-6263. No accessories are needed and SoftMax® Pro Software. Description: The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research. Plus 384. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. S. 40266 Email: drugdiscoverytech@lifetech. Perkin Elmer Victor X2 Multimode Microplate Plate Reader 2030-0020 w/ PC. Detection of KASP genotyping reactions For the second test, the validation kit contained 36 known samples, including 33 DNA samples and 3 no-template controls (NTC). ELISA Workflow Using the EMax plus Microplate Reader and MultiWash+ Microplate Washer. Fluorescence Microplate Reader Comparison Chart. Fig 2: Enzymatic response of synthetic compounds. 1 up to 3600 sec. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the operation of such Molecular Devices equipment, software, reagents, and consumables. Installation Qualification (IQ)the iD5 reader’s filter system. Devices PathCheck® Sensor—the only patented technology available that measures the depth (optical pathlength) ofWe would like to show you a description here but the site won’t allow us. Viewing results on your new SpectraMax iD3 Multi-Mode Microplate Reader. sensitivity or performance. 00. Plate reader. SpectraMax® Microplate Readers and SoftMax® Pro Software. Narrow bandwidths deliver increased accuracy and enhanced peak resolution. The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. 0. For more sample throughput, the system can be easily integrated into a full robotic The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modesRobust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter. For a higher degree of automation, the Automation Vendor Partners Program has streamlined the integration of our microplate reader s with all This UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. 00. 0077 OD 0. Figure 10: The Plate section Template Editor, showing the series set up. The instrument is designed to regulate the temperature of the plate chamber from 5°C above ambient to 45°C. The adapter is required for optimum performance with standard 96-well and 384-. In an analogous manner, the same sample names were assigned to a CuvetteSet section. EMax. Enable more assay conditions Define individual reagents to be delivered to each well reducing reagent consumption and allowing for more assay conditions to be explored on a single microplate. 0. 2 only) SpectraMax M-series Microplate readers. Accurate quantitation The optical design of the SpectraMax 340PC384 reader truly mimics a dual. Reader from Molecular Devices can run both standard spectrophotometer and microplate reader applications on the same instrument. Our extensive SpectraMax® line of user-friendly microplate readers – the industry’s most cited brand – provide great flexibility for labs conducting applications ranging from ELISAs to nucleic acid and protein quantitation, and include absorbance, fluorescence, luminescence and more SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. Uncontrolled Copy for Training and Reference Purposes Only . That’s why Molecular Devices offers the following services: Installation qualification (IQ), operational qualification (OQ), preventive maintenance (PM), and repair coverage. 7°C and the bottom to 36. The plate was covered and mixed on an orbital shaker for five minutes and then placed in a SpectraMax i3x reader and mixed. The following components are located on the back panel of the SpectraMax and VersaMax readers: • Power switch:[The Multi-Mode Validation Plate offers additional testing of TRF, HTRF, and fluorescence polarization read modes on FilterMax™ F3/F5 and SpectraMax® i3x, iD5, and Paradigm® microplate readers. Get the most out of SoftMax Pro. (1) Repertoires of SARS-CoV-2 epitopes targeted by antibodies vary according to severity of COVID-19 Virulence December 31, 2022 David J. ORDER. Devices’ StakMax® Microplate Stacker in a matter of minutes and . This document is copyright protected and any reproduction of this document, in whole or. Laboratory automation workflows can help. GxP. e. begin reading microplates with seven mouse clicks. Better absorbance accuracy. Mode Reader with the StakMax Microplate Handling System from Molecular Devices. Assay flexibility. ] Example of document to evaluate if the reader . Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). Step 1: Turn on your microplate reader. Page 28 Chapter 5: Read Modes and Read Types 1. Our specialized plate readers for absorbance, fluorescence, and luminescence detection provide optimal sensitivity. Molecular Devices' microplate readers. The optical system uses two scanning monochromators so you can determine optimal excitation and emission settings, resulting in. Supplier: Molecular Devices. Luciferase reporter assays are widely used because of their sensitive detection capacity and broad dynamic range. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. They feature options and modules to address your specific research including objectives, filters, imaging modes, and environmental. When the user applies a set temperature of 37°C, the top is heated to 37. , Sunnyvale, CA 94089, USA, Jin_Liao@moldev. Monthly Plate Verification Check (Absorbance Validation Plate) Note: Monthly Plate Verification Check is maintained by Quality Control. To maintain confidence in the standards, Molecular Devices recommends having validation plates recertified at one-to Molecular Devices for recertification at our ISO 17025 accredited lab are disassembled, cleaned, calibrated, and Luminescence is the emission of light by a substance as a result of a chemical reaction called chemiluminescence or an enzymatic reaction called bioluminescence. We therefore gathered salient. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. Increase productivity with patented microplate reader technologies. They are widely used in research, drug discovery, [1] bioassay validation, quality control and manufacturing processes in the pharmaceutical. Experimental details and customization of the group table. SpectraMax Paradigm Multi-Mode Safety Guide From plate readers to high-content imagers, Molecular Devices has the instruments you need to advance your discovery. The adapter is required for optimum performa nce with standard 96-well and 384-. • Analyze data in a single plate, across multiple plates, or between different groups. Cellular Imaging Systems: Molecular Devices offers various imaging solutions for your research needs. SpectraDrop High-Throughput Screening (HTS) Kit. The lower limit of detection (LLD) was defined as the level of. Note that this procedure is only for the SpectraMax L model. #0200-6263) UltraVision™ Plate 384 (Brooks Life Sciences cat. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. Microplate Stacker and Washer. Published Dec 16, 2020 | Updated Dec 16, 2020. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. Step 2: Launch SoftMax Pro Software. Page 2 SpectraMax ® SpectraMax ® Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. Ensure data accuracy of your reader. Important things to consider when purchasing a multimode microplate reader. Table 2 summarizes the results for the uniformity test. #Flex3) FlexStation 3 8-channel Pipettor Head (Molecular Devices cat. Monday to Friday 7am - 5pm Pacific Time. A plate containing two columns each of 75 µL, 100 µL, 150 µL, 200 µL, 250 µL, or 300 µL of yellow buffer solution was read at 426 nm on a SpectraMax microplate reader. The Gemini XPS Reader can be integrated with Molecular Devices’ StakMax® Microplate Stacker in a matter of minutes and begin reading microplates with seven mouse clicks. This page contains the. The whole plate can be detect in a single run with ease and flexible. Microplate Readers. SpectraMax M series, Gemini, and SpectraMax iDx readers have xenon flash lamps,. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. The drawing shows a standard microplate of 15 mm height covered with a lid. 1. #6590-0108) Fluorescence detection using the SpectraMax iD5 reader’s monochromators for wavelength selection was compared to that using a filter pair specific for the Alexa594 tracer. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the. Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, CA 94089 USA Tel: 408. Read one sample or up to 384 in a single plate read using any standard cuvette, 12 x 75 mm test tube, or 96- or 384-well microplate. Page 16 FilterMax F3 and FilterMax F5 Multi-Mode Microplate Readers User Guide To exchange an excitation or emission filter in a slide: 1. D SpectraMax ® M2 SpectraMax ® M2 e Multimode Plate Readers User Guide Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089. 1. Citations on Google Scholar. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11. Page 1 SpectraMax M3, M4, M5, and M5 ® Multi-Mode Microplate Readers User Guide 0112-0115 F July 2010. 0) OD (0. This can increase throughput and efficiency, thus saving time and space in the lab. 2. #4TI-0655) Microplates. Supplier: Molecular Devices The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes. Explore our fully-integrated,. For 40 years, we have partnered with scientists to help them achieve landmark discoveries. Part Number Description 1-2100-0548 USB computer connection cable, 3 meter (9. FilterMax F3 and F5 Multi-Mode Microplate Readers are filter-based microplate readers offering fluorescence polarization, time-resolved fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. For top-reading microplate readers (SpectraMax M2, M5, FlexStation II, and FlexStation 3) is it fine to leave the plate lid on? Will the excitation light get reflected off the lid in an unpredictable way?Sunnyvale, California -- October 5, 2017. The user guide also covers the software features, the maintenance procedures, and the technical specifications of the readers. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a. We would like to show you a description here but the site won’t allow us. to that of dedicated single-mode readers. Versa Max. Get any of the iD series microplate readers with up to 25% discount off the current list price in their basic configuration and receive a two-year warranty for commercial or a three-year warranty for Academic customers. The VersaMax™ Microplate Reader from Molecular Devices bridges the gap between the affordability of filter-based readers and the flexibility of monochromator-based systems. Optimize wavelengths-protocols from Molecular Devices are on computer desktop. Step 4: Add detection antibody – Enzyme-conjugated detection antibody binds to a second site on the. It is more inert and reduces compound interference. 1. The touchscreen interface provides integrated instrument control, data display, and the ability to export results for statistical data analysis. 0 . 304 signal level saturation. Molecular Devices also offers Digital IQ/OQ/PM. 2. Molecular Devices, LLC Optical System: Inquire; Measuring Time: White light - 96-well/ 384 well microplate: <2 min Fluorescence - 96-well/ 384 well microplate: <8 min. The plate reader optics are tunable for absorbance, fluorescence intensity, fluorescence polarization, and time resolved fluorescence assays (TRF). Next generation SpectraMax® absorbance microplate readers. Turn on Plate Reader and wait for it to complete its start-up routine. BMG Labtech - THERMOstar. Testing can be done in the user’s lab on their own schedule. Published Dec 16, 2020 | Updated Nov 08, 2023. Category 2 and 3 microplate readers use gradient heating as well. #S1207. Molecular Devices SpectraMax 384 Plus Microplate Reader. S. Molecular Devices microplate readers. DOCUMENTATION. 747. A microplate reader with fluorescence intensity (FI) detection uses a light source, usually a Xenon flash lamp or LED, to excite a fluorophore (fluorescent molecule) at a particular wavelength. #4TI-0214) UltraVision Plate 96 (Brooks Life. A: adipocytes; B: fibroblasts; C: no cells (uptake reagent only). The FilterMax™ F3 features absorbance (visible), fluorescence intensity - FI (top reading), and glow luminescence for 96- to 384-well plates. Other features and benefits include: Eliminate changing out filters. Microscopes and Microplate Instrumentation. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Accurate and sensitive detection of nucleic acids and proteins are critical to many experiments. Our 15 years of experience means we understand these instruments as well as the manufacturer, and it allows us to provide top-quality service. FlexStation® 3 Multi-Mode Microplate Reader. . #6590-0121) SpectraMax i3x® Multi-Mode Microplate Reader (Molecular Devices cat. 150 μL of MTT solvent was then added to each well to solubilize the formazan product, and the plate was placed on a shaker for 15 minutes. An ampicillin-resistant E. SpectraMax iD5 Multi-Mode Microplate Reader, a hybrid monochromator- and filter-based, five-mode plate reader with automatic filter identification and western blot capability. On LabX buyers can find a variety of SpectraMAX models for sale: 190, 384, 340 and the SpectraMAX M series. Molecular Devices training courses and tutorials. Multimode Microplate Readers. Products : SpectraMax M2/M2e reader, SpectraMax M5/M5e reader, Gemini XPS reader, FlexStation II (Obsolete), Gemini EM reader, FlexStation 3 reader. White plates reflect the light, enabling even low levels of Luminescence to be detected. Calcium flux was measured with a FlexStation 3 Microplate Reader using the Flex (fast kinetic) read type. PBS was removed, and cell pellets were. First choose Read Mode ‘FL’ and Read Type ‘Endpoint’, and then select. This multi plate reader is excellent to work with for checking the U. With various on-board features and. +44-118-944-8000. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. PathCheck sensor corrects optical pathlength & pipetting variation. This can increase throughput and efficiency, thus saving time and space in the lab. generation SpectraTest ABS2 Absorbance Validation Plate. Plate section without PathCheck applied. If it looks fine, then proceed. Extended linear dynamic range. SpectraMax® Microplate Readers and SoftMax® Pro Software. 100 μL of each endotoxin standard was added to triplicate wells of an LAL Reagent Grade Multi-well Plate. Analyst GT Microplate Reader: Luminescence height = 1 mm, max integration as 1,000,000 μsec. Gemini XPS & EM readers measure fluorescence on a variety of sample formats from 6- to 384-well. Click on each for more information. 50/50. It this case, the EMax Plus Microplate Reader was the default. performs within its specifications. *Purchase is subject to Molecular Devices standard terms and conditions of sale. This can increase throughput and efficiency, thus saving time and space in the lab. Combined with the analysis capabilities of SoftMax® Pro Software, we provide quantitation in an easy-to-read, user-customizableThe SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. SoftMax Pro Software. * Filters (diameter) or optics shown as center wavelength/bandwidth (nm) & dichroic mirrors shown as cutoff wavelength (nm); Contact Molecular Devices for information on ordering filters & dichroic mirrors suitable with the microplate readers listed above. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of. ABS2 Validation Plate Protocol Installation InstructionsEndpoint, dual read, kinetic, fast kinetic. (a) Chemical structures of 1 (HADP) and its analogues 2–4. Two. Wide photometric range Temperature-regulated Thermal control and vertical shake Microsoft Windows-based software Automatic mixing Five-second read per plate Photometric and kinetic analysis mode The Molecular Devices ThermoMax is an efficient, temperature-regulated, 96 well microplate reader. If flexibility becomes important in a growing lab supporting a variety of projects, the decision might come down to whether. 1. W×D×H. Tunable, filterless flexibility The VersaMax reader uses a grating monochromator to select the wavelength, so the exact wavelength needed can be selected for every assay. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements. Turn the reader on and secure the USB cable. book Page 1 Tuesday, July 6, 2010 2:08 PM SpectraMax Microplate Readers. Microplate Readers. Create custom fluorescence assays without having to purchase expensive filter sets. Microplates for Molecular Devices Microplate Readers – Photometric Accuracy Fluorescence Intensity Luminescence Intensity (0. Small but mighty absorbance plate reader •ffordable monochromator-based plate reader with linearity A of up to 3 OD • orks with 96- and 384-well plates as well as cuvettes W. The assay is easy to set up and the range of potential analytes is vast, but the assay procedure is time consuming and labor intensive. Instrument settings can be saved as a protocol file. SpectraMax and VersaMax Plate Readers Operator’s Manual — 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Fluorescence Microplate Reader Comparison Chart. SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers. Step 1: Open the SoftMax Pro IMAP FP protocol. A standard spectrophotometer measures the absorbance of one sample at a time. User selects appropriate wells for samples and control using plate configuration tool. Compact designs. #6590-0118) Filter 665/10 (Molecular Devices cat. Remove the lamp screw (on the right side of the lamp, as shown in the diagram) then gently pull the connecting wires to remove the lamp from the body of the instrument. SpectraMax M2 & SpectraMax M2e Multi-mode Plate Readers User Guide 0112-0102 Rev. With three modes of detection for full spectrum absorbance, fluorescence and luminescence, the SpectraMax® Mini reader . Turn the reader on and secure the USB cable. 1 sec. together with a Molecular Devices plate. The SpectraMax M5e Micoplate Reader is the standard for UV/Visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-well and 384-well microplates. visible absorbance, fluorescence. Unplug the power cable from the back of the instrument. Support. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The adapter is required for optimum performance with standard 96-well and 384-. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent. These tools can be used in conjunction with SoftMax® Pro Software validation package and IQ/OQ/PQ validation protocols for FDA 21 CFR Part 11 compliance. #SpectraMax L Config) Methods. Instrument settings can be saved as a protocol file. 1700 Online: Sales Offices USA/Canada 800. In addition, the iD5 reader measures TRF and FP and can be expanded to include TR-FRET, HTRF®, BRET, dual luciferase reporter assays with injectors, and western blot detection. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. After reading the ELISA plate on both readers, each standard curve was nearly identical. Microplate Reader Comparison Charts. Page 1 ™ VERSAmax Tunable Microplate Reader Operator’s Manual for Instruments with Serial Numbers Beginning with BN Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0084 Rev. Multimode microplate readers deliver both performance and economy. Detection cartridge modularity allows for easy addition of new read modes within minutes without. LMax and LMax II plate readers use photon counting mode similar to the SpectraMax L reader. The SpectraMax® QuickDrop™ UV-Vis Spectrophotometer quantifies very small amounts of DNA, RNA, oligos, and proteins. SpectraMax Microplate Readers. The variations include adjustable speed and volume, adjustable aspiration speed and time, adjustable soak times, and three modes of. 0 . Description Figure 1. Eight filters enable visible-wavelength absorption measurement applications such as protein quantification, cell viability and ELISA. ensure your reader is thoroughly tested against specifications we consider critical to achieving quality results. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. . ®Plate chamber design of the SpectraMax category 1 microplate readers. Please click on the links below to view the Microplate Reader Comparison Tables. Page 1 of 8 . It then uses the pre-defined settings for the specified instrument to build a protocol. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. 9. This five-mode microplate reader with automatic filter identification and western blot capability is the complete. 0112-0126B 8 Chapter1:Introduction TheSpectraMax®340PC384,SpectraMax®190,andVersaMax™microplate. SpectraMax ABS and ABS Plus Absorbance ELISA Microplate Readers. 1-10 sec. Molecular Devices launches custom organoid line expansion service using unique bioprocess technology for high-throughput applications. 1. Highly customizable low volume applications Molecular Devices unique SpectraDrop™ Micro-The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. IMAP assays are based on binding of. Recombinant protein expression Nucleocapsid. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of detection allowing a single piece of equipment to perform a wider range of assays. 312 gain calibration failed. Shop now. The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. Transfected cells in the 6-well plate were trypsinized, divided into ten aliquots per well, pelleted at 1500 rpm for 5 minutes, and washed once with PBS. 0. 2. • Fluorescence Polarization (FP-FLUO) Detection Cartridge (Molecular Devices cat. Preventive Maintenance (PM) Each plate reader or washer is calibrated, inspected, and lubricated—any potential issuesSpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. #S1205. Fluorescence Microplate Reader Comparison Chart. Detection modalities include absorbance (UV-Vis Abs), fluorescence intensity (FI), luminescence (Lumi), and all have. Detection modalities include absorbance (UV-Vis Abs) and fluorescence intensity (FI). FlexIII. ; Page 2 Molecular Devices Corporation warrants this product against defects in material or workmanship as follows:. 24 deep well. Molecular Devices SpectraMax M5 Multi-Mode Microplate Reader. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. ¨Automated microplate readers reduce manual manipulation and ensure consistent operation. We would like to show you a description here but the site won’t allow us. Step 3: Wash microplate – Unbound material is washed away, leaving only the antigen of interest. Select Start>Control panel>Device Manager. 6% for the on-board optics (monochromators). The plate was then re-read on the plate reader (Read B). As Glazer explains, “Our readers have software that analyzes data as it’s acquired from a plate—doing both at the same time. The plates were then read with the SpectraMax M3 plate reader (Molecular Devices, Sunnyvale, CA) to obtain absorbance readings at 570 nm. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. As new assays are developed or your needs change, simply add a detection cartridge like ScanLater™ Western Blot System or the SpectraMax® MiniMax™ Imaging Cytometer option as opposed to buying a brand new system. : 8 Oct 12 Page 1 of 10 Setup Guide on the Molecular Devices SpectraMax® M5/M5e Microplate Reader Have a question? Contact our Technical Support Team NA: 800-955-6288 or INTL: 760-603-7200 ext. The system comes with a single plate loading adapter and a choice of magazines for 20, 40 or 50 SBS-standard 96- and 384-well. 더 많은. Molecular Devices SpectraMax ® i3x MultiMode Microplate Reader, in combination with luminescent assays for cell viability and cytotoxicity, provides a sensitive and rapid way to measure the number of viable cells in culture and quantify the cytotoxic effects of experimental treatment. Sale price $3,495. Step 3. 58% for the detection module and 2. #Flex3) Methods. Superior optical system includes a xenon flash lamp and features. Page 1 of 8 . The microplate (containing standards) was incubated at 37ºC in the preheated SpectraMax ABS Plus reader for 10 minutes. What are the physical and environmental operating specifications for SpectraMax UV/Vis and Gemini plate readers? (With extra info on backup power UPS choices). San Jose, California, June 5, 2018. It features a modular design that allows users to add or change detection cartridges according to their needs. The reader measures both flat and round 96-well microplates and ensures accuracy by automatic lamp calibration prior to. The VersaMax Absorbance Microplate Reader allows for selection of up to two wavelengths at a time for absorbance detection in the visible wavelength range (340 nm - 850 nm). 0. SpectraTest Multi-Mode Validation Plate User Guide SpectraTest Validation Plates and SoftMax Pro Software reader validation protocols. Restart the system. The SpectraMax iD5 reader uses the HTRF detection system, which includes an Enhanced TRF Module and filters for excitation and emission. The temperature in the plate chamber is ambient and isothermal. 25 to 3. 0 software Download page. Learn about the SpectraMax i3x Multi-Mode Plate Reader capabilities and options in this short video. Select Start>Control panel>Device Manager. 1. 1 second up to 30 seconds. 1-2 sec. Microplate Reader Shelf. We would like to show you a description here but the site won’t allow us. Below is the list of Molecular Devices microplate readers and liquid handling instruments that are of out-of-production and are now considered obsolete. SpectraMax ABS Plus Microplate Reader (Molecular Devices cat. 118. The guide also covers the software features, the specifications, and the accessories of the systems. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Learn more about how absorbance is measured, and some key applications that utilize absorbance. Fluorescence Filter with holder 565nm BW 30nm (Molecular Devices cat. FlexStation® 3 Multi-Mode Microplate Reader. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. Search. Use form “26003-02: Molecular Devices Plate Reader Plate Calibration Form” to record material and equipment information. SoftMax® Pro 7. For biochemical assays, the washer removes unbound material and unreacted reagents. 7°C (gradient heating design). Contains a revised proprietary masking dye with higher light extinction in the extracellular solution to significantly reduce background and increase signal window. Feb 22, 2023Fast and compact absorbance microplate readers for a wide range of assays without the use of filtersSpectraMax M3, M4, M5, and M5e Microplate Reader User Guide Connecting Instrument Cables The power cord and USB cable connect to the ports on the rear of the instrument. This assay kit is optimized for SpectraMax microplate readers from Molecular Devices, with a pre. Read the plate on the SpectraMax M5, M5e, or FlexStation 3 reader using the optimized settings shown above. The trademarks used herein are the property of Molecular Devices, LLC or their respective owners. Data analysis. FlexStation 3 Multimode Microplate Reader (Molecular Devices Cat. Flexibility to read a variety of plate formats - SpectraMax® Absorbance Microplate Readers can run both cuvette-based and microplate reader applications on the same instrument. SpectraMax Readers have the most complete level of product validation and compliance. Standalone instrument with touchscreen and built-in sample ports for small volumes and cuvettes. 0 OD for our latest microplate readers, SpectraMax® iD3, iD5, ABS, and ABS Plus readers, and up to 2. 0 . Here, we demonstrate that with Molecular Devices SpectraMax® microplate readers and the Quant-iT PicoGreen assay, users can reliably measure concentrations as low as 50 pg/mL of double-stranded DNA. , prior to compound addition) from the cells. ®ELISA (Enzyme-linked Immunosorbent Assay) is one of the most popular quantitative methods to detect a target antigen such as toxin or foreign substance within a sample. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. Cellular Imaging Systems. These plate readers in the list below, are no longer included with the new SoftMax Pro 6 reader menu. It provides ready-to-run protocols, analysis algorithms, and 21 different curve fit options. HTRF-compatible SpectraMax Multi-Mode Readers. Other readers with cell imaging include PerkinElmer’s EnSight™ Multimode Plate Reader and Molecular Devices’ SpectraMax i3x Multi-Mode Microplate Reader. Complete plate reader solution for all your research needs For over 40 years, we have partnered with scientists to expand the boundaries of their research. By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. The SpectraMax® M3 Multi-Mode Microplate Reader features dual monochromatic optics to allow the widest range of applications to be utilized for bioresearch procedures, without the need to change filters. “Sensitivity is pretty much clutch for luminescence,” says Daly. Avoid losing high signal. FAST AND ACCURATE METERTECH Microplate Reader $3,650. FLIPR Tetra systems are fluorescence- and luminescence-based microplate readers with an integrated 1536-, 384- or 96-pipettor. With the novel filter system, assays such as hydrogen peroxide detection assays and IRantibody-binding assays can be run confidently with Molecular Devices. A plate reader’s software may offer automated analysis using protocols configured for a particular assay. Next generation SpectraMax® absorbance microplate readers. 0) at 37 °C, fluorescence intensity was recorded on a Spectramax M5 Multimode plate reader. The StakMax system integrated with Molecular Devices microplate readers and AquaMax washers provides the walk-away automation for up to 50 microplates. Explore more about the SpectraMax i3x Multi-Mode Plate Reader, a user-upgradeable detection modules allow you to expand the capabilities of your microplate reader when your research requires it. Find regional support. The upgradeable SpectraMax M5 Multi-Mode Microplate Reader comes with the standard five modes of detection with the upgrade option to add HTRF at a later time. The SpectraMax ABS. FlexIII. Built-in Help function answers your most frequently asked questions. (“Molecular Devices”) equipment, software, reagents, and consumables to use in the. 3T3 L1 adipocytes were plated at 50,000 cells/well in 100 µL of DMEM/FBS in a 96-well plate and incubated at 37°C for five hours,Lower variance: Normalize your plate reader assay data to the number of cells in each well.